Crystallization of human oxyhaemoglobin from poly(ethylene glycol) solutions.

نویسندگان

  • M J Grabowski
  • A M Brzozowski
  • Z S Derewenda
  • T Skarźnński
  • M Cygler
  • A Stepień
  • A E Derewenda
چکیده

Crystals of human oxyhaemoglobin were obtained from poly(ethylene glycol) solutions. Spectroscopic and spectrophotometric measurements on the solutions during crystallization and on the dissolved crystals indicate that the method yields stable crystals of oxyhaemoglobin. Preliminary X-ray studies showed that the crystals obtained are isomorphous with those of deoxyhaemoglobin obtained from poly(ethylene glycol) solutions [Ward, Wishner, Lattman & Love (1975) J. Mol. Biol. 98, 161-177].

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthesis and Thermal Properties of Novel Biodegradable ABCBA Pentablock Copolymers from Poly (Ethylene glycol), L-Lactide and p-Dioxanone

In this work, new biodegradable ABCBA type pentablock copolymers with different mole ratio of L-lactide and PPDO-b-PEG-b-PPDO triblock copolymer were synthesized and characterized. In the first step, PPDO-b-PEG-b-PPDO triblock copolymer was synthesized via a ring-opening polymerization of P-DiOxanone (PDO) monomer with Poly (Ethylene Glycol) (P...

متن کامل

Scattering Study of Conductive-Dielectric Nano/Micro-Grained Single Crystals Based on Poly(ethylene glycol), Poly(3-hexyl thiophene) and Polyaniline

Two types of rod-coil block copolymers including poly(3-hexylthiophene)-block-poly(ethylene glycol) (P3HT-b-PEG) and PEG-block-polyaniline (PANI) were synthesized using Grignard metathesis polymerization, Suzuki coupling, and interfacial polymerization. Afterward, two types of single crystals were grown by self-seeding methodology to investigate the coily and rod blocks in grafted brushes and o...

متن کامل

Preventive Effects of Cannabis Sativaon Renal Calcium Crystallization Induced by Ethylene Glycol in Rats

BACKGROUND: Some herbal plants can prevent kidney stones formation in a dose dependent manner. In present study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycol-induced rats. MATERIALS AND METHODS: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium and PH) have been ...

متن کامل

Semicrystalline poly(vinyl alcohol) films and their blends with poly(acrylic acid) and poly(ethylene glycol) for drug delivery applications

Semicrystalline films of poly(vinyl alcohol) (PVA) were prepared by annealing amorphous PVA films at temperatures above the glass transition temperature of 85°C, thus allowing the macromolecules to form crystallites, which stabilized the films and made them behave as if they were chemically cross-linked. Films were prepared by casting a 15% aqueous PVA solution onto glass slides and annealing t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Biochemical journal

دوره 171 1  شماره 

صفحات  -

تاریخ انتشار 1978